Separation of a plasma phospholipid transfer protein from cholesterol ester/phospholipid exchange protein.

Separation of a plasma phospholipid transfer protein from cholesterol ester/phospholipid exchange protein.

The d > 1.21 g/ml fraction of human plasma stimulates mass transfer of phosphatidylcholine (PC) from egg PC vesicles into plasma high density lipoproteins (HDL) and also exchange of PC and cholesterol esters (CE) between HDL and low density lipoproteins (LDL). By measuring the facilitated transfer of PC from egg PC vesicles into HDL, and the facilitated exchange of cholesterol esters between HD...

Phospholipid transfer protein interacts with and stabilizes ABCA1 and enhances phospholipid and cholesterol efflux from cells

Phospholipid transfer protein-deficient mice absorb less cholesterol.

OBJECTIVE Phospholipid transfer protein (PLTP) plays an important role in lipoprotein metabolism and atherosclerosis. PLTP gene knockout (KO) mice show significant reduction of plasma cholesterol levels. Because small intestine is one of the major tissue expressing PLTP, we hypothesize that PLTP deficient small intestine absorbs less cholesterol, thus contributing to the diminishing of choleste...

Macrophage phospholipid transfer protein deficiency and ApoE secretion: impact on mouse plasma cholesterol levels and atherosclerosis.

OBJECTIVE PLTP and apoE play important roles in lipoprotein metabolism and atherosclerosis. It is known that formation of macrophage-derived foam cells (which highly express PLTP and apoE) is the critical step in the process of atherosclerosis. We investigated the relationship between PLTP and apoE in macrophages and the atherogenic relevance in a mouse model. METHODS AND RESULTS We transplan...

Different locations of cholesteryl ester transfer protein and phospholipid transfer protein activities in plasma.

Activities of cholesteryl ester transfer protein (CETP) and phospholipid transfer protein (PLTP) were measured in plasma of four vertebrate species: man, rabbit, pig, and rat. The activities were measured in the absence and presence of antibodies raised against purified human CETP. PLTP activities were present in all four species with highest values in pig (11.7 +/- 1.2 U/ml) and human plasma (...